The sne mutants were isolated in a screen for suppressors of
the sly1-10 loss-of-function phenotype (Strader
et al., 2004). Germination rates, fertility
and plant height are complemented in these plants.
However, this publication does not mention the effect of
the sne mutation on flowering time.
SNE is a highly conserved gene in plants.
It is 471 bp long and encodes for a
predicted polypeptide of 157 amino-acids.
SNE transcript levels are
high in the stems and flowers, and low
in the seedlings, leaves and green
SNE stimulates RGA degradation:
In the complete absence of SLY1 (plants expressing
antisens SLY1 transcripts), the plants have a
phenotype. The expression of SNE is unaffected in these
The sne mutant phenotype is actually caused by
over-expression of SNE. Plants over-expressing SNE under
the control of the 35S promoter also show complementation
of the sly1-2 or sly1-10 loss-of-function
mutant phenotypes, and contain very low levels of RGA protein. This means that
can functionally complement SLY1 as a negative
regulator of RGA (Strader
et al., 2004).